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Tuesday, October 25, 2011

Applications of Plant Tissue Culture : Auricularia polytricha

Ear Mushroom Cultivation  has long been recognized as a delicious and nutritious food. Many also have grown. (mushroom cultivation)
There are two classes of ear mushrooms. The first type is thin, for example, Auricularia amicula Jodal Schors a reddish brown or white juciformisyang Tremellaria. Both types are thick, for example Aricularia polytricka (Month) Sacc. Blackish brown.
These inputs into the water extracts 20gr jelly, 20gr dextrose or glucose, and yeast 5gr while in stir with a spoon slowly until completely dissolved. This dough back over the stove to cook it, stirring until boiling. New subsequently poured into Erlenmeyer or glass bottles, then bottle corked with cotton or alumunium foil. The bottles were in sterilization in an autoclave at a temperature of 120-125o C for 15-20 minutes. After cooling, media to be ready in use as a tissue culture growth media. (mushroom cultivation)






Seed Sources Ear Mushrooms
The next stage is the stage of supplying fresh mushrooms as a source of seed, which is healthy there is no pest or pests attacked the former, thick, and fairly easy. Sources of seed fungus in the morning quotation. (mushroom cultivation)
Furthermore, mushrooms cleaned of dirt with sterile water or a solution of alcohol 40%, then split into 2 parts sheet. By using an angled needle OSE taken little part in the parts of the fungus to spread in the media to spread their seeds. During 5-6 days the media who have contracted it dieramkan at 22-26o C, until there seems miseliumnya growth. Mycelium has yet to be purified again in the new media. Also after it should dieramkan at 22-26o C for 5 days. Generally 5 days later the growth of mycelium had covered nearly three-fourths of the surface of agar media, so it's ready to be transferred to a medium powder. (mushroom cultivation)
Media powder is a mixture of 100 parts of softwood sawdust, 5 parts of bran, 0.5 parts CaCO3, 0.5 parts plaster, and 70% water is sterilized in an autoclave for 2-3 hours at a temperature of 115-120 ° C, once inserted into the plastic bag. The new culture can be moved kemedia pure powder. The result, after dieramkan in the incubator temperature 22-26 ° C, for 2-3 weeks will appear in the media powder tumbuhi fungal mycelium. Media prepared powder was used as a seed oyster mushrooms
Working order mushroom seed production
(How to tissue culture)
1. Potatoes cut into pieces for yeast dextrose-agar medium.
2. Aquades boiled potatoes.
3. Water filtered potato extract.
4. Air potato jelly extract, yeast, dextrose cooked again.
5. The mixture was poured into a glass bottle.
6. Sterilization in an autoclave
7. Cooled.
8. Mushroom seed selection.
9. Mushrooms cut some
10. Sliced ​​mushrooms 2, taken part in the.
11. Transferred to agar media.
12. Being a pure culture in order to
13. Preparation of media powder, sawdust CaCO3 + casts.
14. Inserted in a plastic bag
15. Powder media in sterilization
16. Pure cultures on media that dinonkulasikan powder
17. Stored until it grows hifanya, ready to use as seed.


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